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Image Search Results
Journal: bioRxiv
Article Title: The formin FMNL2 plays a role in the response of melanoma cells to substrate stiffness
doi: 10.1101/2024.12.13.628356
Figure Lengend Snippet: . Substrate modulus affects actin stress fiber orientation in A2058 cells. (A) 2D orientational order parameter S quantifying average orientation of F-actin with respect to the cell Feret diameter. Increasing substrate stiffness increases the 2D order parameter. ° indicates values outside of 2 standard deviations. N=3 (B, C) Representative images of the cells analyzed in (A) for control and FMNL2 depleted cells plated on the indicated substrates. Cells were fixed and stained with phalloidin to visualize actin filaments. Scale bar=20 mm (D) Anti-FMNL2 immunoblot confirming the extent of FMNL2 knockdown by transient transfection of siRNA. Tubulin was used as a loading control. (E) Windrose plots showing the orientation of the actin fibers with respect to the cells’ Feret diameter for control and FMNL2 depleted cells plated on 0.2kPa and 64kPa substrates. Increases in substrate modulus and FMNL2 depletion both yield a tighter distribution around 0, indicating increased filament alignment.
Article Snippet: Advanced Biomatrix CytoSoft ® Imaging 24-well Plate 0.2kPa (#5183) and
Techniques: Control, Staining, Western Blot, Knockdown, Transfection
Journal: bioRxiv
Article Title: The formin FMNL2 plays a role in the response of melanoma cells to substrate stiffness
doi: 10.1101/2024.12.13.628356
Figure Lengend Snippet: Substrate modulus affects actin stress fiber orientation in A2058 cells. (A) 2D orientational order parameter S quantifying average orientation of F-actin with respect to the cell Feret diameter. Increasing substrate stiffness increases the 2D order parameter. FMNL2 knockdown results are shown for FMNL2 depletion using siRNA duplex 2. Controls cells are as shown in . ° indicates values outside of 2 standard deviations. N=3. (B, C) Representative images of the cells analyzed in (A) for control and FMNL2 depleted cells plated on the indicated substrates. Cells were fixed and stained with phalloidin to visualize actin filaments. Scale bar=20 µ m. (D) Windrose plots showing the orientation of the actin fibers with respect to the cells’ Feret diameter for control and FMNL2 depleted cells plated on 0.2kPa and 64kPa substrates. Increases in substrate modulus and FMNL2 depletion both yield a tighter distribution around 0, indicating increased filament alignment.
Article Snippet: Advanced Biomatrix CytoSoft ® Imaging 24-well Plate 0.2kPa (#5183) and
Techniques: Knockdown, Control, Staining